Plastic fiber optics for micro-imaging of fluorescence signals in living cells

Takashi Sakurai; Mitsuo Natsume; Kowa Koida

doi:10.1117/12.2076928

2 March 2015 Plastic fiber optics for micro-imaging of fluorescence signals in living cells

Takashi Sakurai, Mitsuo Natsume, Kowa Koida

Proceedings Volume 9328, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIII; 93281N (2015) https://doi.org/10.1117/12.2076928
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

The fiber-coupled microscope (FCM) enables in vivo imaging at deep sites in the tissues or organs that other optical techniques are unable to reach. To develop FCM-based intravital imaging, we employed a plastic optical fiber (POF) bundle that included more than 10,000-units of polystyrene core and polymethyl methacrylate cladding. Each POF had a diameter of less than 5 μm; the tip of the bundle was less than 0.5 mm wide, and the flexible wire had a length of 1,000 mm. The optical performance of the plastic FCM was sufficient for detection of significant signal changes in an acinus of rat pancreas labeled with a calcium ion–sensitive fluorescent dye. In the future, the potential power of plastic FCM is expected to increase, enabling analysis of structure and organization of specific functions in live cells within vulnerable organs.

Citation Download Citation

Takashi Sakurai, Mitsuo Natsume, and Kowa Koida "Plastic fiber optics for micro-imaging of fluorescence signals in living cells", Proc. SPIE 9328, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIII, 93281N (2 March 2015); https://doi.org/10.1117/12.2076928

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KEYWORDS

Luminescence

Phase only filters

Signal detection

Calcium

Tissue optics

Tissues

Microscopes

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