An automated tool for 3D tracking of single molecules in living cells

L. Gardini; M. Capitanio; F. S. Pavone

doi:10.1117/12.2080378

9 March 2015 An automated tool for 3D tracking of single molecules in living cells

L. Gardini, M. Capitanio, F. S. Pavone

Proceedings Volume 9331, Single Molecule Spectroscopy and Superresolution Imaging VIII; 933117 (2015) https://doi.org/10.1117/12.2080378
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

Since the behaviour of proteins and biological molecules is tightly related to cell’s environment, more and more microscopy techniques are moving from in vitro to in living cells experiments. Looking at both diffusion and active transportation processes inside a cell requires three-dimensional localization over a few microns range, high SNR images and high temporal resolution. Since protein dynamics inside a cell involve all three dimensions, we developed an automated routine for 3D tracking of single fluorescent molecules inside living cells with nanometer accuracy, by exploiting the properties of the point-spread-function of out-of-focus Quantum Dots bound to the protein of interest.

Citation Download Citation

L. Gardini, M. Capitanio, and F. S. Pavone "An automated tool for 3D tracking of single molecules in living cells", Proc. SPIE 9331, Single Molecule Spectroscopy and Superresolution Imaging VIII, 933117 (9 March 2015); https://doi.org/10.1117/12.2080378

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