Quantitative phase recovery from asymmetric illumination on an LED array microscope

Lei Tian; Laura Waller

doi:10.1117/12.2078286

11 March 2015 Quantitative phase recovery from asymmetric illumination on an LED array microscope

Lei Tian, Laura Waller

Proceedings Volume 9336, Quantitative Phase Imaging; 93360A (2015) https://doi.org/10.1117/12.2078286
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

Differential phase contrast (DPC) is a quantitative phase imaging technique which measures the sample’s phase derivative by taking two images from complementary asymmetric illumination patterns. Distinct from coherent techniques, DPC relies on partially coherent illumination, providing 2× better lateral resolution, better optical sectioning, and immunity to speckle noise. In this paper, we derive the weak object transfer function to quantify how sample’s phase is converted into our DPC measurements, then develop quantitative inversion methods. Phase reconstructions from single-axis DPC measurements suffer from missing frequencies along the axis of asymmetry. We measure the missing frequency information by taking DPC measurements from other axes. Our phase reconstruction method provides a unified framework for both single and multi-axis DPC measurements. We implement our DPC measurements in real-time and along arbitrary axes of asymmetry by computational illumination on an LED array microscope.

Citation Download Citation

Lei Tian and Laura Waller "Quantitative phase recovery from asymmetric illumination on an LED array microscope", Proc. SPIE 9336, Quantitative Phase Imaging, 93360A (11 March 2015); https://doi.org/10.1117/12.2078286

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