Paper
22 May 2015 Three-dimensional microscopy through liquid-lens axial scanning
Ana Doblas, E. Sánchez-Ortiga, G. Saavedra, J. Sola-Pikabea, M. Martínez-Corral, Po-Yuan Hsieh, Yi-Pai Huang
Author Affiliations +
Abstract
In this contribution we propose the use of a liquid lens (LL) to perform three-dimensional (3D) imaging. Our proposed method consists on inserting the LL at the aperture stop of telecentric microscopes. The sequential depth images of 3D samples are obtained by tuning the focal length of LL. Our experimental results demonstrate that fast-axial scanning of microscopic images is obtained without varying neither the resolution capability nor the magnification of the imaging system. Furthermore, this non-mechanical approach can be easily implemented in any commercial optical microscope.
© (2015) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Ana Doblas, E. Sánchez-Ortiga, G. Saavedra, J. Sola-Pikabea, M. Martínez-Corral, Po-Yuan Hsieh, and Yi-Pai Huang "Three-dimensional microscopy through liquid-lens axial scanning", Proc. SPIE 9495, Three-Dimensional Imaging, Visualization, and Display 2015, 949503 (22 May 2015); https://doi.org/10.1117/12.2177680
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CITATIONS
Cited by 3 scholarly publications.
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KEYWORDS
Molybdenum

Microscopes

3D image processing

Microscopy

Imaging systems

Point spread functions

Relays

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