Angular and spectrally resolved investigations of yeast cells by light scattering microscopy and goniometric measurements

Julian Stark; Dennis Müller; Steffen Nothelfer; Alwin Kienle

doi:10.1117/12.2183789

15 July 2015 Angular and spectrally resolved investigations of yeast cells by light scattering microscopy and goniometric measurements

Julian Stark, Dennis Müller, Steffen Nothelfer, Alwin Kienle

Author Affiliations +

Proceedings Volume 9537, Clinical and Biomedical Spectroscopy and Imaging IV; 953718 (2015) https://doi.org/10.1117/12.2183789
Event: European Conferences on Biomedical Optics, 2015, Munich, Germany

Abstract

Spectrally and angular resolved light scattering from yeast cells was studied with a scattering microscope and a goniometer. Different cell models were investigated with help of analytical solutions of Maxwell's equations. It was found that extraction of precise morphological and optical cellular properties from the measured scattering patterns and phase functions requires more sophisticated cell models than standard Mie theory.

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Julian Stark, Dennis Müller, Steffen Nothelfer, and Alwin Kienle "Angular and spectrally resolved investigations of yeast cells by light scattering microscopy and goniometric measurements", Proc. SPIE 9537, Clinical and Biomedical Spectroscopy and Imaging IV, 953718 (15 July 2015); https://doi.org/10.1117/12.2183789

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