Single-cell diffraction tomography with optofluidic rotation about a tilted axis

Paul Müller; Mirjam Schürmann; Chii J. Chan; Jochen Guck

doi:10.1117/12.2191501

25 August 2015 Single-cell diffraction tomography with optofluidic rotation about a tilted axis

Paul Müller, Mirjam Schürmann, Chii J. Chan, Jochen Guck

Proceedings Volume 9548, Optical Trapping and Optical Micromanipulation XII; 95480U (2015) https://doi.org/10.1117/12.2191501
Event: SPIE Nanoscience + Engineering, 2015, San Diego, California, United States

Abstract

Optical diffraction tomography (ODT) is a tomographic technique that can be used to measure the three-dimensional (3D) refractive index distribution within living cells without the requirement of any marker. In principle, ODT can be regarded as a generalization of optical projection tomography which is equivalent to computerized tomography (CT). Both optical tomographic techniques require projection-phase images of cells measured at multiple angles. However, the reconstruction of the 3D refractive index distribution post-measurement differs for the two techniques. It is known that ODT yields better results than projection tomography, because it takes into account diffraction of the imaging light due to the refractive index structure of the sample. Here, we apply ODT to biological cells in a microfluidic chip which combines optical trapping and microfluidic flow to achieve an optofluidic single-cell rotation. In particular, we address the problem that arises when the trapped cell is not rotating about an axis perpendicular to the imaging plane, but is instead arbitrarily tilted. In this paper we show that the 3D reconstruction can be improved by taking into account such a tilted rotational axis in the reconstruction process.

Conference Presentation

Citation Download Citation

Paul Müller, Mirjam Schürmann, Chii J. Chan, and Jochen Guck "Single-cell diffraction tomography with optofluidic rotation about a tilted axis", Proc. SPIE 9548, Optical Trapping and Optical Micromanipulation XII, 95480U (25 August 2015); https://doi.org/10.1117/12.2191501

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