Super-resolution imaging with mid-IR photothermal microscopy on the single particle level

Zhongming Li; Masaru Kuno; Gregory Hartland

doi:10.1117/12.2188337

20 August 2015 Super-resolution imaging with mid-IR photothermal microscopy on the single particle level

Zhongming Li, Masaru Kuno, Gregory Hartland

Proceedings Volume 9549, Physical Chemistry of Interfaces and Nanomaterials XIV; 954912 (2015) https://doi.org/10.1117/12.2188337
Event: SPIE Nanoscience + Engineering, 2015, San Diego, California, United States

Abstract

Photothermal microscopy has achieved single molecule sensitivity. However, the analytes are usually restricted to be natural absorbers in the visible light region. Mid-infrared (MIR) imaging, on the other hand, provides a wealth of information, but encounters difficulties of diffraction-limited spatial resolution and scarcity of ideal detectors. Here we present Mid-IR photothermal heterodyne imaging (MIR-PHI) microscopy as a high sensitivity, super-resolution mid-IR imaging technique. In MIR-PHI, a tunable Mid- IR pulsed laser at 150 kHz is used to excite a micron sized particle. Energy relaxation creates a temperature gradient around the particle, changing the refractive index of the surrounding solvent and creating a thermal lens. A collinear, counter propagating probe beam (a 532 nm CW laser) is modified by the thermal lens and generates a super-resolution photothermal image. We studied 1.1 μm polystyrene beads at the single particle level using this technique. Various solvents with different heat capacities and refractive indices are tested for the best image contrast. The wide applicability and potentially high sensitivity of this technique make it promising for biological imaging and identification.

Conference Presentation

Citation Download Citation

Zhongming Li, Masaru Kuno, and Gregory Hartland "Super-resolution imaging with mid-IR photothermal microscopy on the single particle level", Proc. SPIE 9549, Physical Chemistry of Interfaces and Nanomaterials XIV, 954912 (20 August 2015); https://doi.org/10.1117/12.2188337

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available