Complexity of genetic sequences modified by horizontal gene transfer and degraded-DNA uptake

George Tremberger Jr.; S. Dehipawala; A. Nguyen; E. Cheung; R. Sullivan; T. Holden; D. Lieberman; T. Cheung

doi:10.1117/12.2186550

11 September 2015 Complexity of genetic sequences modified by horizontal gene transfer and degraded-DNA uptake

George Tremberger Jr., S. Dehipawala, A. Nguyen, E. Cheung, R. Sullivan, T. Holden, D. Lieberman, T. Cheung

Proceedings Volume 9606, Instruments, Methods, and Missions for Astrobiology XVII; 96060H (2015) https://doi.org/10.1117/12.2186550
Event: SPIE Optical Engineering + Applications, 2015, San Diego, California, United States

Abstract

Horizontal gene transfer has been a major vehicle for efficient transfer of genetic materials among living species and could be one of the sources for noncoding DNA incorporation into a genome. Our previous study of lnc- RNA sequence complexity in terms of fractal dimension and information entropy shows a tight regulation among the studied genes in numerous diseases. The role of sequence complexity in horizontal transferred genes was investigated with Mealybug in symbiotic relation with a 139K genome microbe and Deinococcus radiodurans as examples. The fractal dimension and entropy showed correlation R-sq of 0.82 (N = 6) for the studied Deinococcus radiodurans sequences. For comparison the Deinococcus radiodurans oxidative stress tolerant catalase and superoxide dismutase genes under extracellular dGMP growth condition showed R-sq ~ 0.42 (N = 6); and the studied arsenate reductase horizontal transferred genes for toxicity survival in several microorganisms showed no correlation. Simulation results showed that R-sq < 0.4 would be improbable at less than one percent chance, suggestive of additional selection pressure when compared to the R-sq ~ 0.29 (N = 21) in the studied transferred genes in Mealybug. The mild correlation of R-sq ~ 0.5 for fractal dimension versus transcription level in the studied Deinococcus radiodurans sequences upon extracellular dGMP growth condition would suggest that lower fractal dimension with less electron density fluctuation favors higher transcription level.

Citation Download Citation

George Tremberger Jr., S. Dehipawala, A. Nguyen, E. Cheung, R. Sullivan, T. Holden, D. Lieberman, and T. Cheung "Complexity of genetic sequences modified by horizontal gene transfer and degraded-DNA uptake", Proc. SPIE 9606, Instruments, Methods, and Missions for Astrobiology XVII, 96060H (11 September 2015); https://doi.org/10.1117/12.2186550

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