Fluorescence spectroscopy incorporating a ratiometric approach for the diagnosis and classification of urothelial carcinoma

Suresh Anand; Riccardo Cicchi; Alfonso Crisci; Gabriella Nesi; Marco Carini; Francesco Saverio Pavone

doi:10.1117/12.2208587

29 February 2016 Fluorescence spectroscopy incorporating a ratiometric approach for the diagnosis and classification of urothelial carcinoma

Suresh Anand, Riccardo Cicchi, Alfonso Crisci, Gabriella Nesi, Marco Carini, Francesco Saverio Pavone

Author Affiliations +

Proceedings Volume 9689, Photonic Therapeutics and Diagnostics XII; 96891A (2016) https://doi.org/10.1117/12.2208587
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

The current most popular clinical method for the screening of urothelial carcinoma is white light cystoscopy. This method has inherent disadvantages making a strong genesis towards developing more powerful diagnostic techniques. Laser induced intrinsic fluorescence spectroscopy has been studied as an adjunct to current methods for the detection of tumors. This technique allows real time results based on the changes in spectral profile between normal and tumor tissues. We conducted a pilot study based on fluorescence spectroscopy at two wavelengths 378 and 445 nm excitation for the differentiation of urothelial carcinoma. At both the excitation wavelengths, the measured fluorescence signal showed an increased intensity at wavelengths greater than 520 nm. In addition, the emission profile showed modulation at 580 nm which is due to the reabsorption of emitted fluo- rescence due to hemoglobin. Additionally, we developed a tissue characterizing algorithm, based on fluorescence intensity ratios, F510/F600 and F520/F580 at 378 and 445 nm excitation wavelengths respectively. Further, the results were correlated with the pathologists assessment of urothelial carcinoma. This ratiometric classification algorithm yielded 81% sensitivity and 83% specificity at 378 nm and while at 445 nm excitation we achieved a sensitivity and specificity of 85% and 86% for classifying normal and tumor bladder tissues. In this study we have demonstrated the potential of a simple ratiometric algorithm based on fluorescence spectroscopy could be an alternative tool to tissue biopsy. Furthermore, this technique based fiber-based fluorescence spectroscopy could be integrated into an endoscopy system for use in the operating room.

Conference Presentation

Citation Download Citation

Suresh Anand, Riccardo Cicchi, Alfonso Crisci, Gabriella Nesi, Marco Carini, and Francesco Saverio Pavone "Fluorescence spectroscopy incorporating a ratiometric approach for the diagnosis and classification of urothelial carcinoma", Proc. SPIE 9689, Photonic Therapeutics and Diagnostics XII, 96891A (29 February 2016); https://doi.org/10.1117/12.2208587

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available