Structural and functional measurements of fertilized mouse oocytes with combined high-resolution OCT and inverted microscope
(Conference Presentation)

Karol Karnowski; Anna Ajduk; Maciej Wojtkowski; Maciej Szkulmowski

doi:10.1117/12.2214736

26 April 2016 Structural and functional measurements of fertilized mouse oocytes with combined high-resolution OCT and inverted microscope (Conference Presentation)

Karol Karnowski, Anna Ajduk, Maciej Wojtkowski, Maciej Szkulmowski

Author Affiliations +

Proceedings Volume 9697, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XX; 96972B (2016) https://doi.org/10.1117/12.2214736
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

We present a comprehensive imaging methodology for 3D structural and functional measurements of fertilized mouse oocytes. In contrary to methods used for mouse zygote imaging so far OCT provides 3D data without z axis movement of sample or objective lens. Furthermore, complex scanning protocols used in this study give access to different scales of repetition times and thus may become a tool for investigation of a different dynamic processes. Additionally, proposed scanning approach via variety of statistic operations can be used to enhance the quality of structural images. OCT system capabilities are presented and compared to standard microscopy. With a single 3D measurements one can extract 3D structure of the oocytes as well as en-face images that correspond to both bright and dark field microscopy. As an example of dynamic oocyte imaging pronuclei motion during development is presented. Limitations and possibilities of the new system are discussed.

Conference Presentation

Citation Download Citation

Karol Karnowski, Anna Ajduk, Maciej Wojtkowski, and Maciej Szkulmowski "Structural and functional measurements of fertilized mouse oocytes with combined high-resolution OCT and inverted microscope (Conference Presentation)", Proc. SPIE 9697, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XX, 96972B (26 April 2016); https://doi.org/10.1117/12.2214736

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available