Forster resonance energy transfer in the system of human serum albumin-xanthene dyes

V. I. Kochubey; A. B. Pravdin; A. G. Melnikov; I. Konstantinova; I. V. Alonova

doi:10.1117/12.2229604

21 April 2016 Forster resonance energy transfer in the system of human serum albumin-xanthene dyes

V. I. Kochubey, A. B. Pravdin, A. G. Melnikov, I. Konstantinova, I. V. Alonova

Proceedings Volume 9917, Saratov Fall Meeting 2015: Third International Symposium on Optics and Biophotonics and Seventh Finnish-Russian Photonics and Laser Symposium (PALS); 991710 (2016) https://doi.org/10.1117/12.2229604
Event: Saratov Fall Meeting 2015, 2015, Saratov, Russian Federation

Abstract

The processes of interaction of fluorescent probes: eosin and erythrosine with human serum albumin (HSA) were studied by the methods of absorption and fluorescence spectroscopy. Extinction coefficients of probes were determined. Critical transfer radius and the energy transfer efficiency were defined by fluorescence quenching of HSA. Analysis of the excitation spectra of HSA revealed that the energy transfer process is carried out mainly between tryptophanyl and probes.

Citation Download Citation

V. I. Kochubey, A. B. Pravdin, A. G. Melnikov, I. Konstantinova, and I. V. Alonova "Forster resonance energy transfer in the system of human serum albumin-xanthene dyes", Proc. SPIE 9917, Saratov Fall Meeting 2015: Third International Symposium on Optics and Biophotonics and Seventh Finnish-Russian Photonics and Laser Symposium (PALS), 991710 (21 April 2016); https://doi.org/10.1117/12.2229604

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KEYWORDS

Energy transfer

Luminescence

Proteins

Absorption

Energy efficiency

Resonance energy transfer

Fluorescence resonance energy transfer

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