Computational illumination for 3D phase microscopy
(Conference Presentation)

Laura Waller

doi:10.1117/12.2235984

4 November 2016 Computational illumination for 3D phase microscopy (Conference Presentation)

Laura Waller

Proceedings Volume 9956, Ultrafast Nonlinear Imaging and Spectroscopy IV; 995610 (2016) https://doi.org/10.1117/12.2235984
Event: SPIE Optical Engineering + Applications, 2016, San Diego, California, United States

Abstract

This talk will describe new computational microscopy methods for high pixel-count 3D images. We describe two setups employing illumination-side and detection-side aperture coding of angle (Fourier) space for capturing 4D phase-space (e.g. light field) datasets with fast acquisition times. Using a multi-slice forward model and compressive sensing inspired algorithms, we develop efficient 3D reconstruction methods for both incoherent and coherent imaging models, with robustness to scattering and aberrations. Experimentally, we achieve real-time 3D intensity and phase capture with high resolution across a large volume. Such computational approaches to optical microscopy add significant new capabilities to commercial microscopes without significant hardware modification. We will discuss applications in 3D microscopy and all-optical neural activity tracking.

Conference Presentation

Citation Download Citation

Laura Waller "Computational illumination for 3D phase microscopy (Conference Presentation)", Proc. SPIE 9956, Ultrafast Nonlinear Imaging and Spectroscopy IV, 995610 (4 November 2016); https://doi.org/10.1117/12.2235984

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KEYWORDS

Microscopy

3D modeling

3D acquisition

Algorithm development

3D image processing

Imaging spectroscopy

Coherence imaging

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