Laboratory-based x-ray phase-contrast tomography enables 3D virtual histology

Mareike Töpperwien; Martin Krenkel; Felix Quade; Tim Salditt

doi:10.1117/12.2246460

16 September 2016 Laboratory-based x-ray phase-contrast tomography enables 3D virtual histology

Mareike Töpperwien, Martin Krenkel, Felix Quade, Tim Salditt

Proceedings Volume 9964, Advances in Laboratory-based X-Ray Sources, Optics, and Applications V; 99640I (2016) https://doi.org/10.1117/12.2246460
Event: SPIE Optical Engineering + Applications, 2016, San Diego, California, United States

Abstract

Due to the large penetration depth and small wavelength hard x-rays offer a unique potential for 3D biomedical and biological imaging, combining capabilities of high resolution and large sample volume. However, in classical absorption-based computed tomography, soft tissue only shows a weak contrast, limiting the actual resolution. With the advent of phase-contrast methods, the much stronger phase shift induced by the sample can now be exploited. For high resolution, free space propagation behind the sample is particularly well suited to make the phase shift visible. Contrast formation is based on the self-interference of the transmitted beam, resulting in object-induced intensity modulations in the detector plane. As this method requires a sufficiently high degree of spatial coherence, it was since long perceived as a synchrotron-based imaging technique. In this contribution we show that by combination of high brightness liquid-metal jet microfocus sources and suitable sample preparation techniques, as well as optimized geometry, detection and phase retrieval, excellent three-dimensional image quality can be obtained, revealing the anatomy of a cobweb spider in high detail. This opens up new opportunities for 3D virtual histology of small organisms. Importantly, the image quality is finally augmented to a level accessible to automatic 3D segmentation.

Citation Download Citation

Mareike Töpperwien, Martin Krenkel, Felix Quade, and Tim Salditt "Laboratory-based x-ray phase-contrast tomography enables 3D virtual histology", Proc. SPIE 9964, Advances in Laboratory-based X-Ray Sources, Optics, and Applications V, 99640I (16 September 2016); https://doi.org/10.1117/12.2246460

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