Multiplexing drug-target engagement and metabolic imaging in HER2-positive tumor xenograft models

Alena Rudkouskaya; Jason T. Smith; Xavier R. Intes; Margarida Barroso

doi:10.1117/12.2607308

7 March 2022 Multiplexing drug-target engagement and metabolic imaging in HER2-positive tumor xenograft models

Alena Rudkouskaya, Jason T. Smith, Xavier R. Intes, Margarida Barroso

Proceedings Volume PC11938, Visualizing and Quantifying Drug Distribution in Tissue VI; PC1193809 (2022) https://doi.org/10.1117/12.2607308
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Macroscopic fluorescence lifetime FRET imaging (MFLI-FRET) presents a much-needed analytical tool to non-invasively quantify drug-receptor engagement in tumors and other organs in preclinical studies. We demonstrate the specificity and sensitivity of MFLI-FRET for direct and robust measurement of trastuzumab-HER2 target engagement in various types of breast and ovarian cancer tumor xenograft models. Simultaneous metabolic imaging with IRDye 800 CW 2-DG reveals that intracellular delivery of drug is associated with 2-DG lifetime and likely reflects tumors’ microenvironment and perfusion state.

Conference Presentation

Citation Download Citation

Alena Rudkouskaya, Jason T. Smith, Xavier R. Intes, and Margarida Barroso "Multiplexing drug-target engagement and metabolic imaging in HER2-positive tumor xenograft models", Proc. SPIE PC11938, Visualizing and Quantifying Drug Distribution in Tissue VI, PC1193809 (7 March 2022); https://doi.org/10.1117/12.2607308

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