PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
Obtaining high signal to noise ratio is challenging in wide-field two photon microscopy and one must ensure the mouse brain can be imaged safely under high laser power. Here, we demonstrated a simultaneous thermal imaging and two photon imaging technique. The maximum temperature of the cortex was below 39°C using 400mW laser power with a 5 x 5mm field of view. Together with the brain activities under hind paw stimulation and at rest, we argued that high laser power for wide-field two-photon imaging can potentially be used while keeping the temperature under safety limit.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
The alert did not successfully save. Please try again later.
Hunter Banks, Shengxuan Chen, Jonathan Bumstead, Lindsey Brier, Annie Bice, Seana Gaines, Joseph Culver, "Simultaneous functional and thermal imaging of mouse cortex," Proc. SPIE PC11946, Neural Imaging and Sensing 2022, PC119460W (28 April 2022); https://doi.org/10.1117/12.2608942