Label-free trace detection of bio-molecules by liquid interface assisted surface-enhanced Raman scattering using microfluidic chip

Shi Bai; Xueli Ren; Kotaro Obata; Yoshihiro Ito; Koji Sugioka

doi:10.1117/12.2609497

7 March 2022 Label-free trace detection of bio-molecules by liquid interface assisted surface-enhanced Raman scattering using microfluidic chip

Shi Bai, Xueli Ren, Kotaro Obata, Yoshihiro Ito, Koji Sugioka

Author Affiliations +

Proceedings Volume PC11955, Microfluidics, BioMEMS, and Medical Microsystems XX; PC1195503 (2022) https://doi.org/10.1117/12.2609497
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Trace detection of bio-molecules with large molecular weight using surface-enhanced Raman scattering (SERS) method is still challenging, because the troublesome treatment of SERS substrate using coupling or cross-linking agents is required. In this paper, we apply liquid interface assisted SERS (LI-SERS) method, which provides unique features of collection and self-immobilization of analyte molecules on the SERS substrate, to realize the label-free trace detection of bio-molecules with detection limits of pM ~ fM. Specifically, DNA discrimination and quantitative detection of β-Amyloid (Aβ) in trace-concentration are demonstrated to illustrate the ultrahigh sensitivity and versatility of the LI-SERS method.

Conference Presentation

Citation Download Citation

Shi Bai, Xueli Ren, Kotaro Obata, Yoshihiro Ito, and Koji Sugioka "Label-free trace detection of bio-molecules by liquid interface assisted surface-enhanced Raman scattering using microfluidic chip", Proc. SPIE PC11955, Microfluidics, BioMEMS, and Medical Microsystems XX, PC1195503 (7 March 2022); https://doi.org/10.1117/12.2609497

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