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The aim of this study was to develop a microfluidic-powered optical platform to real-time monitor microbial biofilm formation at the single-cell level under a precisely controlled laminar flow condition and to rapidly evaluate the cleaning effect of laser irradiation on a mature microbial biofilm. A 405-nm laser light was used to evaluate the cleaning effects on both mature biofilms. The results show that Staphylococcus aureus biofilm was reduced by 80% in population, which was 20% higher than that of Candida albicans biofilm. A further study will be conducted in a poly-microbial biofilm commonly causing urinary tract infections.
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Van Nam Tran, Won Han, Fazlurrahman Khan, Van Gia Truong, Joong Ho Shin, Young Mog Kim, Hyun Wook Kang, "Rapid evaluations of laser cleaning efficacy on mature biofilm with microfluidic-powered optical platform," Proc. SPIE PC11958, Optical Interactions with Tissue and Cells XXXIII; and Advanced Photonics in Urology, PC119580F (7 March 2022); https://doi.org/10.1117/12.2612147