Structured unknown-illumination based super-resolved label free imaging

Gal Chen; Abhijit Sanjeev; Vismay Trivedi; Javier Garcia; Ricardo Rubio; Zeev Zalevsky

doi:10.1117/12.2608555

2 March 2022 Structured unknown-illumination based super-resolved label free imaging

Gal Chen, Abhijit Sanjeev, Vismay Trivedi, Javier Garcia, Ricardo Rubio, Zeev Zalevsky

Proceedings Volume PC11972, Label-free Biomedical Imaging and Sensing (LBIS) 2022; PC1197204 (2022) https://doi.org/10.1117/12.2608555
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Time multiplexing is a super resolution technique that sacrifices time to overcome the resolution reduction obtained because of diffraction. In structured illumination super-resolved imaging, high resolution and time changing patterns are projected on top of an object and a set of low resolution images are captured with the low quality imaging system. The set of low resolution images are digitally decoded with decoding patterns that are based on the high resolution projected encoding patterns. In conventional structured-illuination approaches, the projected encoding patterns need to be a priori known in order to be used in the decoding process. In this presentation, we will describe an enhancement of structured illumination approach towards label free imaging while obtaining the super resolved result without the need of requiring the a priori knowledge on the projected encoding patterns.

Conference Presentation

Citation Download Citation

Gal Chen, Abhijit Sanjeev, Vismay Trivedi, Javier Garcia, Ricardo Rubio, and Zeev Zalevsky "Structured unknown-illumination based super-resolved label free imaging", Proc. SPIE PC11972, Label-free Biomedical Imaging and Sensing (LBIS) 2022, PC1197204 (2 March 2022); https://doi.org/10.1117/12.2608555

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