Towards quantification of actin rotation by nonbleaching nanoscale imaging

Xintao Zhao; John Gregg; Guangjie Cui; Zhijia Zhang; Di Zu; Somin Eunice Lee

doi:10.1117/12.2610430

3 March 2022 Towards quantification of actin rotation by nonbleaching nanoscale imaging

Xintao Zhao, John Gregg, Guangjie Cui, Zhijia Zhang, Di Zu, Somin Eunice Lee

Proceedings Volume PC11976, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIX; PC119760C (2022) https://doi.org/10.1117/12.2610430
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Cell migration is an essential cellular process that help cells to develop complex organisms, organs, and tissues, arrange cells into specialized architecture and help organize the nervous system. Nanoscale imaging has the potential to provide new insight into the mechanics of cell migration. However, quantification over time in nanoscale imaging remains difficult. Here, we visualize cell migration by non-bleaching nanoscale imaging. We present a set of quantitative metrics - length, branching, gaps, distribution, and curvature - to quantitatively analyze cell migration at the nanoscale over time.

Conference Presentation

Citation Download Citation

Xintao Zhao, John Gregg, Guangjie Cui, Zhijia Zhang, Di Zu, and Somin Eunice Lee "Towards quantification of actin rotation by nonbleaching nanoscale imaging", Proc. SPIE PC11976, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIX, PC119760C (3 March 2022); https://doi.org/10.1117/12.2610430

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