Effect of Cell density and laser exposure duration on threshold temperature for photothermal damage

Jason Cui; Julia R. Wieland; Nathaniel J. Pope; Gary D. Noojin; Michael L. Denton

doi:10.1117/12.2657280

27 March 2023 Effect of Cell density and laser exposure duration on threshold temperature for photothermal damage

Jason Cui, Julia R. Wieland, Nathaniel J. Pope, Gary D. Noojin, Michael L. Denton

Proceedings Volume PC12377, Optical Interactions with Tissue and Cells XXXIV; PC123770B (2023) https://doi.org/10.1117/12.2657280
Event: SPIE BiOS, 2023, San Francisco, California, United States

Conference Poster

Abstract

In support of previous cell culture work, we wanted to determine if cell density has an effect on the temperature at which cells die. We used the method of microthermography because it provided threshold temperatures. By not using laser dose, we avoided complications from varying absorptivity and diffusivity of samples. Microthermography looks at temperature at the boundary between live and dead cells using a high-speed mid-IR camera and high-magnification optics. The temperature at the boundary of cell death is independent of the irradiance and represents the true biological threshold regardless of the size of damaged area. Using a 2-um laser, we explored whether the boundary of cell death, and thus the damage rate process, is dependent on cell confluency.

Citation Download Citation

Jason Cui, Julia R. Wieland, Nathaniel J. Pope, Gary D. Noojin, and Michael L. Denton "Effect of Cell density and laser exposure duration on threshold temperature for photothermal damage", Proc. SPIE PC12377, Optical Interactions with Tissue and Cells XXXIV, PC123770B (27 March 2023); https://doi.org/10.1117/12.2657280

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