Spinning arrayed optofluidic imaging for large-scale single-cell image-based analysis (Conference Presentation)

Man Lai Wong; Dickson M. D. Siu; Sum Lau; Kenneth K. Y. Wong; Kevin K. M. Tsia

doi:10.1117/12.2655551

16 March 2023 Spinning arrayed optofluidic imaging for large-scale single-cell image-based analysis (Conference Presentation)

Man Lai Wong, Dickson M. D. Siu, Sum Lau, Kenneth K. Y. Wong, Kevin K. M. Tsia

Author Affiliations +

Proceedings Volume PC12390, High-Speed Biomedical Imaging and Spectroscopy VIII; PC123900G (2023) https://doi.org/10.1117/12.2655551
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

We report an arrayed optofluidic imaging platform that allows multiplexed image-based cell assay at ultrahigh imaging throughput. The assay platform, consisting of 96 fluidic sample chambers arranged in a circular symmetry, operates in a reconfigurable spinning motion synchronized with an ultrafast laser-scanning microscope (a line-scan rate >10 MHz). Based on a stable through-focus spinning mechanism, the assay platform allows ultralarge field-of-view imaging (18.8 cm2, >160 Gpixels) with high image fidelity and subcellular resolution (in both the bright-field and quantitative phase image contrasts). We further validated that the continuous spinning operation has minimal impact on the cell morphology and viability.

Conference Presentation

Citation Download Citation

Man Lai Wong, Dickson M. D. Siu, Sum Lau, Kenneth K. Y. Wong, and Kevin K. M. Tsia "Spinning arrayed optofluidic imaging for large-scale single-cell image-based analysis (Conference Presentation)", Proc. SPIE PC12390, High-Speed Biomedical Imaging and Spectroscopy VIII, PC123900G (16 March 2023); https://doi.org/10.1117/12.2655551

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