Multicore fiber endoscopy for 3D rotation of cancer cells

Juergen W. Czarske; Nektarios Koukourakis; Jiawei Sun

doi:10.1117/12.2678494

12 August 2023 Multicore fiber endoscopy for 3D rotation of cancer cells

Juergen W. Czarske, Nektarios Koukourakis, Jiawei Sun

Proceedings Volume PC12622, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials VI; PC1262205 (2023) https://doi.org/10.1117/12.2678494
Event: SPIE Optical Metrology, 2023, Munich, Germany

Abstract

Fiber-optic dual-beam trap is an emerging method for studying biophysical properties of biological cells and organoids. Nevertheless, current optical manipulation is often limited to single-axis cell rotation. We introduce an innovative dual beam trap, utilizes multicore fibers to enable precise control of cell rotation about all three axes. This is achieved through the development of a physics-informed neural network that generates tailored light fields in the trapping region via the multicore fiber, allowing real-time holographic control of the optical force. By leveraging the capability of 3D cell rotation, our system enables 3D optical diffraction tomography with full spatial frequency coverage, eliminating the missing cone problem for cancer cells.

Conference Presentation

Citation Download Citation

Juergen W. Czarske, Nektarios Koukourakis, and Jiawei Sun "Multicore fiber endoscopy for 3D rotation of cancer cells", Proc. SPIE PC12622, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials VI, PC1262205 (12 August 2023); https://doi.org/10.1117/12.2678494

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