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13 March 2024 Temperature effects on the uptake of photosensitizers in Escherichia coli
Thalita Hellen Nunes Lima, Alessandra Ramos Lima, Kamila Jessie Sammarro Silva, Kate C. Blanco, Francisco Eduardo Gontijo Guimarães, Vanderlei Salvador Bagnato
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Proceedings Volume PC12822, Photonic Diagnosis, Monitoring, Prevention, and Treatment of Infections and Inflammatory Diseases 2024; PC128220B (2024) https://doi.org/10.1117/12.3004525
Event: SPIE BiOS, 2024, San Francisco, California, United States
Abstract
Escherichia coli is responsible for infections in the community and in hospital settings. Photodynamic inactivation (PDI) is an alternative for the treatment of E. coli infections. However, the internalization of photosensitizers (PS), a key element for PDI efficient performance, is still not fully understood. Here, we evaluated thermal and temporal effects on PS uptake in E. coli suspensions. PS internalization estimates were performed by UV-visible absorption spectroscopy and showed influences of temperature and time in PS internalization. We believe that finding ideal conditions for PS uptake in E. coli can improve the PDI process.
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Thalita Hellen Nunes Lima, Alessandra Ramos Lima, Kamila Jessie Sammarro Silva, Kate C. Blanco, Francisco Eduardo Gontijo Guimarães, and Vanderlei Salvador Bagnato "Temperature effects on the uptake of photosensitizers in Escherichia coli", Proc. SPIE PC12822, Photonic Diagnosis, Monitoring, Prevention, and Treatment of Infections and Inflammatory Diseases 2024, PC128220B (13 March 2024); https://doi.org/10.1117/12.3004525
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KEYWORDS
Picosecond phenomena
Oxygen
Bacteria
Absorption spectroscopy
Electrons
Resistance
Spectral response
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