Dual-ratio technique to improve detection depth in diffuse in-vivo fluorescence flow cytometry

Giles Blaney; Fernando Ivich; Angelo Sassaroli; Mark Niedre; Sergio Fantini

doi:10.1117/12.3001842

13 March 2024 Dual-ratio technique to improve detection depth in diffuse in-vivo fluorescence flow cytometry

Giles Blaney, Fernando Ivich, Angelo Sassaroli, Mark Niedre, Sergio Fantini

Proceedings Volume PC12827, Multiscale Imaging and Spectroscopy V; PC1282705 (2024) https://doi.org/10.1117/12.3001842
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Diffuse in-vivo Flow Cytometry (DiFC) is a fluorescence sensing method to detect labeled circulating cells in-vivo non-invasively. The Dual-Ratio (DR) is an optical measurement method that aims to suppress most noise and enhance SNR in deep tissue regions. We investigate DR applied to Near-InfraRed (NIR) DiFC to improve circulating cells’ maximum detectable depth and SNR. Results indicate that two critical conditions control the success of DR DiFC. First, the fraction of noise that DR methods cannot cancel (i.e., non-multiplicative factors) cannot dominate. Second, DR DiFC has an advantage regarding SNR if the spatial distribution of tissue autofluorescence contributors is surface-weighted.

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(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Giles Blaney, Fernando Ivich, Angelo Sassaroli, Mark Niedre, and Sergio Fantini "Dual-ratio technique to improve detection depth in diffuse in-vivo fluorescence flow cytometry", Proc. SPIE PC12827, Multiscale Imaging and Spectroscopy V, PC1282705 (13 March 2024); https://doi.org/10.1117/12.3001842

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KEYWORDS

Signal to noise ratio

In vivo imaging

Flow cytometry

Fluorescence

Tissues

Autofluorescence

Monte Carlo methods

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