Development and validation of a multimodal optical coherence microscopy (OCM) and confocal fluorescence microscope to diagnose ocular surface diseases and tear film disorders

Reddikumar Maddipatla; Samuel R. McCuskey; Brian C. Leonard; Ratheesh K. Meleppat; Maciej M. Bartuzel; Ravi S. Jonnal; Robert J. Zawadzki

doi:10.1117/12.3003069

13 March 2024 Development and validation of a multimodal optical coherence microscopy (OCM) and confocal fluorescence microscope to diagnose ocular surface diseases and tear film disorders

Reddikumar Maddipatla, Samuel R. McCuskey, Brian C. Leonard, Ratheesh K. Meleppat, Maciej M. Bartuzel, Ravi S. Jonnal, Robert J. Zawadzki

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Proceedings Volume PC12834, Multimodal Biomedical Imaging XIX; PC128340R (2024) https://doi.org/10.1117/12.3003069
Event: SPIE BiOS, 2024, San Francisco, California, United States

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Abstract

A multimodal imaging system was developed, integrating swept-source optical coherence microscopy (SS-OCM) with a confocal fluorescence microscope for studying dry eye disease (DED). SS-OCM utilized a 1060 nm central wavelength source, resulting in 7.5 μm axial and 3.4 μm lateral resolution. The fluorescence subsystem employed a 488 nm laser with 2.4 μm lateral resolution. Simultaneous data acquisition at 100 kHz A-scan/pixel was achieved. The system allowed the characterization of the ocular surface's biochemical composition in vivo using fluorescently labeled ligands, facilitating visualization of adhering fluorescent molecules in animal models. Proof-of-concept experiments with a quantum dot-tagged ligand showed potential for improved DED diagnosis and therapeutic response evaluation.

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Reddikumar Maddipatla, Samuel R. McCuskey, Brian C. Leonard, Ratheesh K. Meleppat, Maciej M. Bartuzel, Ravi S. Jonnal, and Robert J. Zawadzki "Development and validation of a multimodal optical coherence microscopy (OCM) and confocal fluorescence microscope to diagnose ocular surface diseases and tear film disorders", Proc. SPIE PC12834, Multimodal Biomedical Imaging XIX, PC128340R (13 March 2024); https://doi.org/10.1117/12.3003069

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