Inverted reflection-mode real-time vibrational microscopy of live cells using microwells-integrated plasmonic metasurfaces

Gennady Shvets

doi:10.1117/12.3012287

13 March 2024 Inverted reflection-mode real-time vibrational microscopy of live cells using microwells-integrated plasmonic metasurfaces

Gennady Shvets

Author Affiliations +

Proceedings Volume PC12839, Biomedical Vibrational Spectroscopy 2024: Advances in Research and Industry; PC128390V (2024) https://doi.org/10.1117/12.3012287
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Water absorption of mid-infrared (MIR) radiation severely limits the options for vibrational spectroscopy of the analytes – including live biological cells – that must be probed in aqueous environments. I will describe a live cell biosensing platform based on metallic nanoantennas fabricated atop elevated dielectric pillars. When cells are cultured on such metasurfaces integrated with standard bottomless microwells, inverted MIR microscopy can be carried out in reflection mode. Results of hyperspectral real-time imaging of live cells during their attachment, spreading, and chemically-induced apoptosis will be presented. Applications to label-free high-content drug screening will be discussed.

Conference Presentation

Citation Download Citation

Gennady Shvets "Inverted reflection-mode real-time vibrational microscopy of live cells using microwells-integrated plasmonic metasurfaces", Proc. SPIE PC12839, Biomedical Vibrational Spectroscopy 2024: Advances in Research and Industry, PC128390V (13 March 2024); https://doi.org/10.1117/12.3012287

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