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13 March 2024 Comparison of microcarrier composition for optical imaging of adherent cells in bioreactor-microcarrier cell cultures
Oscar R. Benavides, Alex J. Walsh
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Proceedings Volume PC12840, Optical Interactions with Tissue and Cells XXXV; PC128400J (2024) https://doi.org/10.1117/12.3001663
Event: SPIE BiOS, 2024, San Francisco, California, United States
Abstract
Visualization-based monitoring provides an empirical and minimally-invasive means for evaluating monolayer cell cultures, but does not translate to three-dimensional microcarrier-based cultures. In toto visualization of cell density and morphology is imperative for producing high quality, high yield cell cultures. Here, the optical properties of commercial polystyrene and custom-fabricated hydrogel microcarriers are compared for compatibility with light-sheet imaging for visualization and enumeration of adherent cells. Additionally, Mie scattering simulations were performed to describe the angular scattering intensity distributions. This study shows the custom hydrogel microcarrier is compatible with in toto non-destructive and non-invasive visualization and monitoring of 3D adherent cell cultures.
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Oscar R. Benavides and Alex J. Walsh "Comparison of microcarrier composition for optical imaging of adherent cells in bioreactor-microcarrier cell cultures", Proc. SPIE PC12840, Optical Interactions with Tissue and Cells XXXV, PC128400J (13 March 2024); https://doi.org/10.1117/12.3001663
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KEYWORDS
Visualization
Mie scattering
Optical imaging
Polystyrene
3D imaging standards
Image visualization
Microscopy
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