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13 March 2024 Investigation on impulsive stimulated Brillouin microscopy for high-speed elastography in hydrogel
David Krause, John Böhm, Leon Liebig, Nektarios Koukourakis, Jürgen Czarske
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Proceedings Volume PC12844, Optical Elastography and Tissue Biomechanics XI; PC128440E (2024) https://doi.org/10.1117/12.3004808
Event: SPIE BiOS, 2024, San Francisco, California, United States
Abstract
In the life sciences, mechanical properties of cells can be used as a diagnostic tool for breast cancer or liver fibrosis, but current techniques require long integration times. The promising impulsive stimulated Brillouin scattering microscopy is an all-optical approach allowing for both a non-invasive and high-resolution measurement. The study reveals that maximizing pulse energy and pulse width achieves high SNR and fast image acquisition, but caution is necessary to stay below the phototoxicity threshold. Imaging at a 10 kHz repetition rate with 20 averages yields good SNR and an imaging speed of 2 ms/pixel, with potential for improvement. A new technique using asymmetric pump focus shows promise in increasing the lateral resolution.
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David Krause, John Böhm, Leon Liebig, Nektarios Koukourakis, and Jürgen Czarske "Investigation on impulsive stimulated Brillouin microscopy for high-speed elastography in hydrogel", Proc. SPIE PC12844, Optical Elastography and Tissue Biomechanics XI, PC128440E (13 March 2024); https://doi.org/10.1117/12.3004808
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KEYWORDS
Microscopy
Signal to noise ratio
Elastography
Hydrogels
Image acquisition
Pulse signals
Signal processing
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