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13 March 2024 Label-free Brillouin endo-microscopy for the quantitative 3D imaging of sub-micrometre biology
Salvatore La Cavera III, Veeren M. Chauhan, William Hardiman, Mengting Yao, Rafael Fuentes-Dominguez, Fernando Perez-Cota, Richard J. Smith, Matt Clark
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Proceedings Volume PC12844, Optical Elastography and Tissue Biomechanics XI; PC128440G (2024) https://doi.org/10.1117/12.3002691
Event: SPIE BiOS, 2024, San Francisco, California, United States
Abstract
In order to realise the clinical potential of Brillouin scattering-based techniques, it is critical to develop an endoscopic probe for measuring elasticity in future in-vivo environments. We have developed a phonon probe which actively injects high amplitude GHz strain pulses into specimens and have demonstrated proof of concept this technique can be used for high resolution 3D imaging. In this talk we show that this new technology is highly applicable to the 3D elasticity imaging of biological tissue from the single-cell scale to multi-cellular organisms and provides a future pathway for the clinical application of in-vivo Brillouin spectroscopy of tissue.
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Salvatore La Cavera III, Veeren M. Chauhan, William Hardiman, Mengting Yao, Rafael Fuentes-Dominguez, Fernando Perez-Cota, Richard J. Smith, and Matt Clark "Label-free Brillouin endo-microscopy for the quantitative 3D imaging of sub-micrometre biology", Proc. SPIE PC12844, Optical Elastography and Tissue Biomechanics XI, PC128440G (13 March 2024); https://doi.org/10.1117/12.3002691
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KEYWORDS
Stereoscopy
Biology
Elasticity
Tissues
Endoscopy
Image resolution
In vivo imaging
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