Towards tomographic molecular imaging via deep ultraviolet (UV) microscopy

Nischita Kaza; Joshua F. Lerner; Paloma Casteleiro Costa; Francisco E. Robles

doi:10.1117/12.3002113

13 March 2024 Towards tomographic molecular imaging via deep ultraviolet (UV) microscopy

Nischita Kaza, Joshua F. Lerner, Paloma Casteleiro Costa, Francisco E. Robles

Proceedings Volume PC12848, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXXI; PC128480E (2024) https://doi.org/10.1117/12.3002113
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Deep Ultraviolet (UV) microscopy enables high-resolution, molecular imaging and typically yields 2D images representing the axial projection of a sample’s 3D absorption onto a plane. In this work, we present a tomographic imaging approach based on multispectral UV microscopy, to visualize complex 3D structural features in samples. We aim to employ through-focus intensity images captured with varying partially coherent and asymmetric illumination patterns to extract the 3D absorption and refractive index (RI) distributions of the sample at distinct UV wavelengths. The recovery procedure relies on solving the inverse scattering problem using the 3D optical transfer function of our microscope.

Conference Presentation

Citation Download Citation

Nischita Kaza, Joshua F. Lerner, Paloma Casteleiro Costa, and Francisco E. Robles "Towards tomographic molecular imaging via deep ultraviolet (UV) microscopy", Proc. SPIE PC12848, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXXI, PC128480E (13 March 2024); https://doi.org/10.1117/12.3002113

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