Fast adaptive optics for deep imaging of biological media in light-sheet and two-photon microscopy

Fabrice Harms; Xavier Levecq; Cynthia Veilly; Mathias Mercier; Alice Guillaume-Manca; Alexandra Fragola; Laurent Bourdieu

doi:10.1117/12.3002671

13 March 2024 Fast adaptive optics for deep imaging of biological media in light-sheet and two-photon microscopy

Fabrice Harms, Xavier Levecq, Cynthia Veilly, Mathias Mercier, Alice Guillaume-Manca, Alexandra Fragola, Laurent Bourdieu

Author Affiliations +

Proceedings Volume PC12851, Adaptive Optics and Wavefront Control for Biological Systems X; PC128510M (2024) https://doi.org/10.1117/12.3002671
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

We report a fast, easy-to-use adaptive optics (AO) method and its implementation in both Light-Sheet and Two-Photon fluorescence microscopy setups. We demonstrate fast (typically 1 second), and accurate aberrations correction at large depths (hundreds of microns) mostly in brain tissue of animal models such as Drosophila, Zebra Fish and mouse. We quantifiy the gain brought by AO in terms of signal (up to x5), contrast, achievable imaging depth and segmentation capability of neuronal structures for both imaging modalities. The approach paves the way to future automated AO-based 3D microscopy systems.

Conference Presentation

Citation Download Citation

Fabrice Harms, Xavier Levecq, Cynthia Veilly, Mathias Mercier, Alice Guillaume-Manca, Alexandra Fragola, and Laurent Bourdieu "Fast adaptive optics for deep imaging of biological media in light-sheet and two-photon microscopy", Proc. SPIE PC12851, Adaptive Optics and Wavefront Control for Biological Systems X, PC128510M (13 March 2024); https://doi.org/10.1117/12.3002671

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