Advancements in quantitative phase Imaging for cell analysis and enzyme activity mapping

Ishan Barman; Piyush Raj; Swati Tanwar

doi:10.1117/12.3008444

13 March 2024 Advancements in quantitative phase Imaging for cell analysis and enzyme activity mapping

Ishan Barman, Piyush Raj, Swati Tanwar

Proceedings Volume PC12852, Quantitative Phase Imaging X; PC128520G (2024) https://doi.org/10.1117/12.3008444
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

In optical imaging, this presentation unveils advancements in two key areas: quantitative phase imaging (QPI) cell segmentation and QPI's application in targeted enzyme activity mapping. QPI complements fluorescence imaging, revealing cell morphology and dynamics without contrast agents. Live-cell 3D tomography enables systematic parameter analysis, free from phototoxicity. Yet, 3D tomogram analysis lags behind imaging speed. Introducing CellSNAP (Cell Segmentation via Novel Algorithm for Phase Imaging), an algorithm that achieves rapid single-core cell segmentation. Addressing clumped cells and interferogram drifts, CellSNAP empowers high-throughput analysis, expanding QPI adoption. Beyond this, QPI's potential in molecular imaging, particularly enzyme activity mapping, is explored. Innovative QPI contrast agents reveal enzyme activity via refractive index modulation, with implications for disease diagnosis and therapeutic assessment.

Conference Presentation

Citation Download Citation

Ishan Barman, Piyush Raj, and Swati Tanwar "Advancements in quantitative phase Imaging for cell analysis and enzyme activity mapping", Proc. SPIE PC12852, Quantitative Phase Imaging X, PC128520G (13 March 2024); https://doi.org/10.1117/12.3008444

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