PPIX delayed and prompt fluorescence for in vivo real time imaging of hypoxic response to burns

Marien Ochoa; Aiping Liu; Matthew S. Reed; Angela Gibson; Brian W. Pogue

doi:10.1117/12.3003544

13 March 2024 PPIX delayed and prompt fluorescence for in vivo real time imaging of hypoxic response to burns

Marien Ochoa, Aiping Liu, Matthew S. Reed, Angela Gibson, Brian W. Pogue

Proceedings Volume PC12854, Label-free Biomedical Imaging and Sensing (LBIS) 2024; PC1285403 (2024) https://doi.org/10.1117/12.3003544
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Real-time visualization of burn severity and hypoxic response is important in understanding wound healing outcomes. Herein we propose a novel approach to macroscopically image burn induced hypoxia through endogenous PpIX delayed fluorescence. The approach is validated in-vivo using athymic nude mice with induced full-thickness burns. PpIX delayed fluorescence imaging was performed longitudinally for 3 days post injury. The localization of PpIX signals was also correlated to Indocyanine Green fluorescence imaging, a technique for blood perfusion and tissue injury in the burn wound. The current results highlight the capability of the approach for real-time in-vivo quantification of hypoxic response to burn wounds.

Conference Presentation

Citation Download Citation

Marien Ochoa, Aiping Liu, Matthew S. Reed, Angela Gibson, and Brian W. Pogue "PPIX delayed and prompt fluorescence for in vivo real time imaging of hypoxic response to burns", Proc. SPIE PC12854, Label-free Biomedical Imaging and Sensing (LBIS) 2024, PC1285403 (13 March 2024); https://doi.org/10.1117/12.3003544

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