Label free full field optical transmission tomography: from nanoscale particles to cells and tissues

Albert Claude Boccara; Samer Alhaddad; Olivier Thouvenin; Martine A. Boccara; Viacheslav Mazlin

doi:10.1117/12.3001154

13 March 2024 Label free full field optical transmission tomography: from nanoscale particles to cells and tissues

Albert Claude Boccara, Samer Alhaddad, Olivier Thouvenin, Martine A. Boccara, Viacheslav Mazlin

Proceedings Volume PC12854, Label-free Biomedical Imaging and Sensing (LBIS) 2024; PC1285409 (2024) https://doi.org/10.1117/12.3001154
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Our past contribution was to introduce a Gouy phase Full Field Optical Transmission technique applied to detect and characterise nanoparticles such as virus, vesicles and nano plastics in terms of size and refractive index. More recently we have adapted this interferometric approach to achieve optical tomography in cells and tissues. We will show improvements in sensitivity and resolution obtained with 20 nm virus as well as with biological tissues using both a static mode (morphology) and dynamic one (metabolic contrast). We discuss the importance of the illumination spatial coherence and compare the results with those obtained with Full Field OCT.

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Citation Download Citation

Albert Claude Boccara, Samer Alhaddad, Olivier Thouvenin, Martine A. Boccara, and Viacheslav Mazlin "Label free full field optical transmission tomography: from nanoscale particles to cells and tissues", Proc. SPIE PC12854, Label-free Biomedical Imaging and Sensing (LBIS) 2024, PC1285409 (13 March 2024); https://doi.org/10.1117/12.3001154

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KEYWORDS

Tissues

Optical transmission

Optical tomography

Particles

Scattering

Phase shifts

Plastics

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