Shortwave infrared photothermal microscopy for millimeter-deep micron-resolution vibrational imaging

Hongli Ni; Yuhao Yuan; Mingsheng Li; Yifan Zhu; Xiaowei Ge; Chinmayee Prabhu Dessai; Ji-Xin Cheng

doi:10.1117/12.3001775

15 March 2024 Shortwave infrared photothermal microscopy for millimeter-deep micron-resolution vibrational imaging

Hongli Ni, Yuhao Yuan, Mingsheng Li, Yifan Zhu, Xiaowei Ge, Chinmayee Prabhu Dessai, Ji-Xin Cheng

Proceedings Volume PC12855, Advanced Chemical Microscopy for Life Science and Translational Medicine 2024; PC1285511 (2024) https://doi.org/10.1117/12.3001775
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Deep-tissue chemical imaging is essential for many biomedical applications. Here, we present a shortwave infrared photothermal (SWIP) microscope for millimeter-deep vibrational imaging with micron-resolution and nanoparticle detection sensitivity. By using short-wave infrared light for overtone excitation of CH bonds and photothermal probing, our modality can reach millimeter deep into scattering phantom and tissue. With a fast digitization method, we showed that in our setting the photothermal signal amplitude is 100 times larger than photoacoustic thus enabling higher sensitivity. SWIP can resolve intracellular lipids in tumor spheroid and layered structure in the liver, skin, and brain tissue.

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Citation Download Citation

Hongli Ni, Yuhao Yuan, Mingsheng Li, Yifan Zhu, Xiaowei Ge, Chinmayee Prabhu Dessai, and Ji-Xin Cheng "Shortwave infrared photothermal microscopy for millimeter-deep micron-resolution vibrational imaging", Proc. SPIE PC12855, Advanced Chemical Microscopy for Life Science and Translational Medicine 2024, PC1285511 (15 March 2024); https://doi.org/10.1117/12.3001775

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KEYWORDS

Infrared imaging

Infrared radiation

Short wave infrared radiation

Vibration

Biomedical optics

Infrared microscopy

Infrared photography

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