Protein microarrays for point-of-care detection from a single drop of blood

Ashutosh Chilkoti

doi:10.1117/12.3005004

13 March 2024 Protein microarrays for point-of-care detection from a single drop of blood

Ashutosh Chilkoti

Proceedings Volume PC12861, Frontiers in Biological Detection: From Nanosensors to Systems XVI; PC1286106 (2024) https://doi.org/10.1117/12.3005004
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

I will describe a point-of-care diagnostic that we have developed, in which all reagents are printed and stored on a “non-fouling”—protein and cell resistant— polymer brush. The D4 assay involves four sequential events: (1) Dispense (droplet of blood); (2) Dissolve (printed reagents on chip); (3) Diffuse (across surface); and (4) Detect (binding event). The D4 assay consists of microarrays of printed on the polymer polymer brush yields quantitative results, with picomolar sensitivity within an hour. All reagents are inkjet-printed and stored on D4 chips, which do not require refrigeration. Upon application of a drop of blood to the D4 chip, analyte capture and detection on the D4 chip occurs automatically, generating an image of fluorescent spots that is imaged by a hand-held detector and quantified by an on-board App. Examples of quantitative dose-response from whole blood will be presented and from patient samples. A plasmonically enhanced version of this assay will also be described.

Conference Presentation

Citation Download Citation

Ashutosh Chilkoti "Protein microarrays for point-of-care detection from a single drop of blood", Proc. SPIE PC12861, Frontiers in Biological Detection: From Nanosensors to Systems XVI, PC1286106 (13 March 2024); https://doi.org/10.1117/12.3005004

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