Magnetic sensing of iron in cytochrome C using diamond quantum sensors

Suvechhya Lamichhane; Cody Schultz; Rupak Timalsina; Ilja Fescenko; Kapildeb Ambal; Sy-Hwang Liou; Rebecca Y. Lai; Abdelghani Laraoui

doi:10.1117/12.3003909

13 March 2024 Magnetic sensing of iron in cytochrome C using diamond quantum sensors

Suvechhya Lamichhane, Cody Schultz, Rupak Timalsina, Ilja Fescenko, Kapildeb Ambal, Sy-Hwang Liou, Rebecca Y. Lai, Abdelghani Laraoui

Author Affiliations +

Proceedings Volume PC12863, Quantum Effects and Measurement Techniques in Biology and Biophotonics; PC128630P (2024) https://doi.org/10.1117/12.3003909
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Iron is an essential yet toxic redox active element that is found in many cells, including neurons and glial cells. Several techniques have been used to quantify iron in neurons and cells; however, most are incapable of high-resolution imaging inside a single cell. Magnetic field sensors based on diamond nitrogen vacancy (NV) centers have emerged as a powerful tool for detecting magnetic signal in iron-containing biological samples with a good combination of spatial resolution and sensitivity. In this study we use NV based T1 relaxometry technique to map iron in cytochrome C (Cyt C) proteins. Cyt C plays an important role in the electron transport chain of mitochondria and it is in the Fe(III) paramagnetic state under ambient conditions. We measure Cyt C under different concentrations and locations of the 10-nm NV doped diamond chip. We show a reduction of the NV T1 from few milliseconds to hundreds of microseconds, explained by the spin noise from the intracellular iron spins in the Cyt C protein. Additionally, we perform imaging of Cyt C proteins on a nanostructured diamond chip.

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Suvechhya Lamichhane, Cody Schultz, Rupak Timalsina, Ilja Fescenko, Kapildeb Ambal, Sy-Hwang Liou, Rebecca Y. Lai, and Abdelghani Laraoui "Magnetic sensing of iron in cytochrome C using diamond quantum sensors", Proc. SPIE PC12863, Quantum Effects and Measurement Techniques in Biology and Biophotonics, PC128630P (13 March 2024); https://doi.org/10.1117/12.3003909

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KEYWORDS

Iron

Diamond

Magnetism

Magnetic sensors

Proteins

Quantum sensing

Quantum sensors

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