High-speed confocal fluorescence imaging with a novel line scanning microscope

Ralf Wolleschensky; Bernhard Zimmermann; Michael Kempe

doi:10.1117/1.2402110

1 November 2006 High-speed confocal fluorescence imaging with a novel line scanning microscope

Ralf Wolleschensky, Bernhard Zimmermann, Michael Kempe

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Journal of Biomedical Optics, Vol. 11, Issue 6, 064011 (November 2006). https://doi.org/10.1117/1.2402110

Abstract

Research in the life sciences increasingly involves the investigation of fast dynamic processes at the cellular and subcellular level. It requires tools to image complex systems with high temporal resolution in three-dimensional space. For this task, we introduce the concept of a fast fluorescence line scanner providing image acquisition speeds in excess of 100 frames per second at 512×512 pixels. Because the system preserves the capability for optical sectioning of confocal systems, it allows us to observe processes with three-dimensional resolution. We describe the principle of operation, the optical characteristics of the microscope, and cover several applications in particular from the field of cell and developmental biology. A commercial system based on the line scanning concept has been realized by Carl Zeiss (LSM 5 LIVE).

©(2006) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Ralf Wolleschensky, Bernhard Zimmermann, and Michael Kempe "High-speed confocal fluorescence imaging with a novel line scanning microscope," Journal of Biomedical Optics 11(6), 064011 (1 November 2006). https://doi.org/10.1117/1.2402110

Published: 1 November 2006

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