Spatially sculpted laser scissors for study of DNA damage and repair

Jared P. Stephens; Samarendra Kumar Mohanty; Suzanne Genc; Xiangduo Kong; Kyoko Yokomori; Michael W. Berns

doi:10.1117/1.3213601

1 September 2009 Spatially sculpted laser scissors for study of DNA damage and repair

Jared P. Stephens, Samarendra Kumar Mohanty, Suzanne Genc, Xiangduo Kong, Kyoko Yokomori, Michael W. Berns

Author Affiliations +

Journal of Biomedical Optics, Vol. 14, Issue 5, 054004 (September 2009). https://doi.org/10.1117/1.3213601

Abstract

We present a simple and efficient method for controlled linear induction of DNA damage in live cells. By passing a pulsed laser beam through a cylindrical lens prior to expansion, an elongated elliptical beam profile is created with the ability to expose controlled linear patterns while keeping the beam and the sample stationary. The length and orientation of the beam at the sample plane were reliably controlled by an adjustable aperture and rotation of the cylindrical lens, respectively. Localized immunostaining by the DNA double strand break (DSB) markers phosphorylated H2AX (γH2AX) and Nbs1 in the nuclei of HeLa cells exposed to the "line scissors" was shown via confocal imaging. The line scissors method proved more efficient than the scanning mirror and scanning stage methods at induction of DNA DSB damage with the added benefit of having a greater potential for high throughput applications.

©(2009) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Jared P. Stephens, Samarendra Kumar Mohanty, Suzanne Genc, Xiangduo Kong, Kyoko Yokomori, and Michael W. Berns "Spatially sculpted laser scissors for study of DNA damage and repair," Journal of Biomedical Optics 14(5), 054004 (1 September 2009). https://doi.org/10.1117/1.3213601

Published: 1 September 2009

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KEYWORDS

Mirrors

Luminescence

Double sideband modulation

Confocal microscopy

Infrared telescopes

Objectives

Microscopes

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