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1 November 2009 Ultrafast pulse-pair control in multiphoton fluorescence laser-scanning microscopy
Arijit Kumar De, Debabrata Goswami
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Journal of Biomedical Optics, Vol. 14, Issue 6, 064018 (November 2009). https://doi.org/10.1117/1.3268440
Abstract
In multiphoton fluorescence laser-scanning microscopy, ultrafast laser pulses [i.e., light pulses having pulse width ≤1 ps (1 ps=10-12 s)] are commonly employed to circumvent the low-multiphoton absorption cross-sections of common fluorophores. Because of the broad overlapping two-photon absorption spectra of fluorophores and the large spectral bandwidth of a short pulse, simultaneous excitation of many fluorophores is common, which justifies a persistent demand for selective excitation of individual fluorophores. We describe the use of pulse-pair excitation with possibilities of controlling molecular fluorescence in laser-scanning microscopy and compare it with coherent control using pulse sequence [De and Goswami, "Coherent control in multiphoton fluorescence imaging," Proc. SPIE 7183, 71832B (2009)].
©(2009) Society of Photo-Optical Instrumentation Engineers (SPIE)
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Arijit Kumar De and Debabrata Goswami "Ultrafast pulse-pair control in multiphoton fluorescence laser-scanning microscopy," Journal of Biomedical Optics 14(6), 064018 (1 November 2009). https://doi.org/10.1117/1.3268440
Published: 1 November 2009
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Cited by 7 scholarly publications.
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KEYWORDS
Multiphoton fluorescence microscopy
Luminescence
Microscopy
Multiphoton microscopy
Pulsed laser operation
Absorption
Modulation
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