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1 September 2010 Enhancing precision in time-domain fluorescence lifetime imaging
Ching-Wei Chang, Mary-Ann Mycek
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Journal of Biomedical Optics, Vol. 15, Issue 5, 056013 (September 2010). https://doi.org/10.1117/1.3494566
Abstract
In biological applications of fluorescence lifetime imaging, low signals from samples can be a challenge, causing poor lifetime precision. We demonstrate how optimal signal gating (a method applied to the temporal dimension of a lifetime image) and novel total variation denoising models (a method applied to the spatial dimension of a lifetime image) can be used in time-domain fluorescence lifetime imaging microscopy (FLIM) to improve lifetime precision. In time-gated FLIM, notable fourfold precision improvements were observed in a low-light example. This approach can be employed to improve FLIM data while minimizing sample light exposure and increasing imaging speed.
©(2010) Society of Photo-Optical Instrumentation Engineers (SPIE)
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Ching-Wei Chang and Mary-Ann Mycek "Enhancing precision in time-domain fluorescence lifetime imaging," Journal of Biomedical Optics 15(5), 056013 (1 September 2010). https://doi.org/10.1117/1.3494566
Published: 1 September 2010
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Cited by 21 scholarly publications.
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KEYWORDS
Fluorescence lifetime imaging
Denoising
Luminescence
Image processing
Imaging systems
Image denoising
Photon counting
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