CONFERENCE PROCEEDINGS
Advanced Search
Home > Journals > J. Biomed. Opt. > Volume 16 > Issue 2 > Article
Open Access
1 February 2011 Nonlinear microscopy, infrared, and Raman microspectroscopy for brain tumor analysis
Tobias Meyer, Norbert Bergner, Christoph Krafft, Denis Akimov, Benjamin Dietzek, Jürgen Popp, Christiane Bielecki, Bernd F. M. Romeike, Rupert Reichart, Rolf Kalff
Author Affiliations +
Journal of Biomedical Optics, Vol. 16, Issue 2, 021113 (February 2011). https://doi.org/10.1117/1.3533268
Abstract
Contemporary brain tumor research focuses on two challenges: First, tumor typing and grading by analyzing excised tissue is of utmost importance for choosing a therapy. Second, for prognostication the tumor has to be removed as completely as possible. Nowadays, histopathology of excised tissue using haematoxylin-eosine staining is the gold standard for the definitive diagnosis of surgical pathology specimens. However, it is neither applicable in vivo, nor does it allow for precise tumor typing in those cases when only nonrepresentative specimens are procured. Infrared and Raman spectroscopy allow for very precise cancer analysis due to their molecular specificity, while nonlinear microscopy is a suitable tool for rapid imaging of large tissue sections. Here, unstained samples from the brain of a domestic pig have been investigated by a multimodal nonlinear imaging approach combining coherent anti-Stokes Raman scattering, second harmonic generation, and two photon excited fluorescence microscopy. Furthermore, a brain tumor specimen was additionally analyzed by linear Raman and Fourier transform infrared imaging for a detailed assessment of the tissue types that is required for classification and to validate the multimodal imaging approach. Hence label-free vibrational microspectroscopic imaging is a promising tool for fast and precise in vivo diagnostics of brain tumors.
©(2011) Society of Photo-Optical Instrumentation Engineers (SPIE)
Citation Download Citation
Tobias Meyer, Norbert Bergner, Christoph Krafft, Denis Akimov, Benjamin Dietzek, Jürgen Popp, Christiane Bielecki, Bernd F. M. Romeike, Rupert Reichart, and Rolf Kalff "Nonlinear microscopy, infrared, and Raman microspectroscopy for brain tumor analysis," Journal of Biomedical Optics 16(2), 021113 (1 February 2011). https://doi.org/10.1117/1.3533268
Published: 1 February 2011
JOURNAL ARTICLE
 10 PAGES
DOWNLOAD PAPER SAVE TO MY LIBRARY
GET CITATION
Lens.org Logo
CITATIONS
Cited by 146 scholarly publications and 3 patents.
Advertisement
Advertisement
RIGHTS & PERMISSIONS
Get copyright permission  Get copyright permission on Copyright Marketplace
KEYWORDS
Tumors
Raman spectroscopy
Brain
Tissues
Second-harmonic generation
Microscopy
Neuroimaging
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top