High-throughput optofluidic system for the laser microsurgery of oocytes

Charlie Chandsawangbhuwana; Linda Z. Shi; Qingyuan Zhu; Michael W. Berns; Mark C. Alliegro

doi:10.1117/1.JBO.17.1.015001

7 February 2012 High-throughput optofluidic system for the laser microsurgery of oocytes

Charlie Chandsawangbhuwana, Linda Z. Shi, Qingyuan Zhu, Michael W. Berns, Mark C. Alliegro

Author Affiliations +

Journal of Biomedical Optics, Vol. 17, Issue 1, 015001 (February 2012). https://doi.org/10.1117/1.JBO.17.1.015001

Abstract

This study combines microfluidics with optical microablation in a microscopy system that allows for high-throughput manipulation of oocytes, automated media exchange, and long-term oocyte observation. The microfluidic component of the system transports oocytes from an inlet port into multiple flow channels. Within each channel, oocytes are confined against a microfluidic barrier using a steady fluid flow provided by an external computer-controlled syringe pump. This allows for easy media replacement without disturbing the oocyte location. The microfluidic and optical-laser microbeam ablation capabilities of the system were validated using surf clam (Spisula solidissima) oocytes that were immobilized in order to permit ablation of the 5 μm diameter nucleolinus within the oocyte nucleolus. Oocytes were the followed and assayed for polar body ejection.

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Charlie Chandsawangbhuwana, Linda Z. Shi, Qingyuan Zhu, Michael W. Berns, and Mark C. Alliegro "High-throughput optofluidic system for the laser microsurgery of oocytes," Journal of Biomedical Optics 17(1), 015001 (7 February 2012). https://doi.org/10.1117/1.JBO.17.1.015001

Published: 7 February 2012

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Cited by 8 scholarly publications.

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KEYWORDS

Microfluidics

Laser ablation

Laser systems engineering

Semiconducting wafers

Control systems

Laser microsurgery

Glasses

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