Label-free subcellular 3D live imaging of preimplantation mouse embryos with full-field optical coherence tomography

Jing-gao Zheng; Tianyuan Chen; Chengming Wang; Ning Tian; Fengying Zhao; Tiancheng Huo; Ning Zhang; Dieyan Chen; Wanyun Ma; Jia-Lin Sun; Ping Xue; Danyu Lu

doi:10.1117/1.JBO.17.7.070503

28 June 2012 Label-free subcellular 3D live imaging of preimplantation mouse embryos with full-field optical coherence tomography

Jing-gao Zheng, Tianyuan Chen, Chengming Wang, Ning Tian, Fengying Zhao, Tiancheng Huo, Ning Zhang, Dieyan Chen, Wanyun Ma, Jia-Lin Sun, Ping Xue, Danyu Lu

Author Affiliations +

Journal of Biomedical Optics, Vol. 17, Issue 7, 070503 (June 2012). https://doi.org/10.1117/1.JBO.17.7.070503

Abstract

Early patterning and polarity is of fundamental interest in preimplantation embryonic development. Label-free subcellular 3D live imaging is very helpful to its related studies. We have developed a novel system of full-field optical coherence tomography (FF-OCT) for noninvasive 3D subcellular live imaging of preimplantation mouse embryos with no need of dye labeling. 3D digitized embryos can be obtained by image processing. Label-free 3D live imaging is demonstrated for the mouse embryos at various typical preimplantation stages with a spatial resolution of 0.7 µm and imaging rate of 24 fps. Factors that relate to early patterning and polarity, such as pronuclei in zygote, shapes of zona pellucida, location of second polar body, cleavage planes, and the blastocyst axis, can be quantitatively measured. The angle between the two second cleavage planes is accurately measured to be 87 deg. It is shown that FF-OCT provides a potential breakthrough for early patterning, polarity formation, and many other preimplantation-related studies in mammalian developmental biology.

Citation Download Citation

Jing-gao Zheng, Tianyuan Chen, Chengming Wang, Ning Tian, Fengying Zhao, Tiancheng Huo, Ning Zhang, Dieyan Chen, Wanyun Ma, Jia-Lin Sun, Ping Xue, and Danyu Lu "Label-free subcellular 3D live imaging of preimplantation mouse embryos with full-field optical coherence tomography," Journal of Biomedical Optics 17(7), 070503 (28 June 2012). https://doi.org/10.1117/1.JBO.17.7.070503

Published: 28 June 2012

ACCESS THE FULL ARTICLE

JOURNAL ARTICLE
4 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY