CONFERENCE PROCEEDINGS
Advanced Search
Home > Journals > J. Biomed. Opt. > Volume 17 > Issue 8 > Article
Open Access
14 May 2012 Optical coherence tomography for live phenotypic analysis of embryonic ocular structures in mouse models
Irina V. Larina, Saba H. Syed, Mary E. Dickinson, Kirill V. Larin, Narendran Sudheendran, Paul A. Overbeek
Author Affiliations +
Journal of Biomedical Optics, Vol. 17, Issue 8, 081410 (May 2012). https://doi.org/10.1117/1.JBO.17.8.081410
Abstract
Mouse models of ocular diseases provide a powerful resource for exploration of molecular regulation of eye development and pre-clinical studies. Availability of a live high-resolution imaging method for mouse embryonic eyes would significantly enhance longitudinal analyses and high-throughput morphological screening. We demonstrate that optical coherence tomography (OCT) can be used for live embryonic ocular imaging throughout gestation. At all studied stages, the whole eye is within the imaging distance of the system and there is a good optical contrast between the structures. We also performed OCT eye imaging in the embryonic retinoblastoma mouse model Pax6-SV40 T-antigen, which spontaneously forms lens and retinal lesions, and demonstrate that OCT allows us to clearly differentiate between the mutant and wild type phenotypes. These results demonstrate that OCTinutero imaging is a potentially useful tool to study embryonic ocular diseases in mouse models.
© 2012 Society of Photo-Optical Instrumentation Engineers (SPIE) 0091-3286/2012/$25.00 © 2012 SPIE
Citation Download Citation
Irina V. Larina, Saba H. Syed, Mary E. Dickinson, Kirill V. Larin, Narendran Sudheendran, and Paul A. Overbeek "Optical coherence tomography for live phenotypic analysis of embryonic ocular structures in mouse models," Journal of Biomedical Optics 17(8), 081410 (14 May 2012). https://doi.org/10.1117/1.JBO.17.8.081410
Published: 14 May 2012
JOURNAL ARTICLE
 5 PAGES
DOWNLOAD PAPER SAVE TO MY LIBRARY
GET CITATION
Lens.org Logo
CITATIONS
Cited by 30 scholarly publications.
Advertisement
Advertisement
RIGHTS & PERMISSIONS
Get copyright permission  Get copyright permission on Copyright Marketplace
KEYWORDS
Optical coherence tomography
Eye
Mouse models
Imaging systems
Biological research
Eye models
Tissues
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top