Diffraction phase microscopy: retrieving phase contours on living cells with a wavelet-based space-scale analysis

Cristina Martinez-Torres; Lotfi Berguiga; Laura Streppa; Elise Boyer-Provera; Laurent Schaeffer; Juan Elezgaray; Alain Arneodo; Françoise Argoul

doi:10.1117/1.JBO.19.3.036007

10 March 2014 Diffraction phase microscopy: retrieving phase contours on living cells with a wavelet-based space-scale analysis

Cristina Martinez-Torres, Lotfi Berguiga, Laura Streppa, Elise Boyer-Provera, Laurent Schaeffer, Juan Elezgaray, Alain Arneodo, Françoise Argoul

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Journal of Biomedical Optics, Vol. 19, Issue 3, 036007 (March 2014). https://doi.org/10.1117/1.JBO.19.3.036007

Abstract

We propose a two-dimensional (2-D) space-scale analysis of fringe patterns collected from a diffraction phase microscope based on the 2-D Morlet wavelet transform. We show that the adaptation of a ridge detection method with anisotropic 2-D Morlet mother wavelets is more efficient for analyzing cellular and high refractive index contrast objects than Fourier filtering methods since it can separate phase from intensity modulations. We compare the performance of this ridge detection method on theoretical and experimental images of polymer microbeads and experimental images collected from living myoblasts.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Cristina Martinez-Torres, Lotfi Berguiga, Laura Streppa, Elise Boyer-Provera, Laurent Schaeffer, Juan Elezgaray, Alain Arneodo, and Françoise Argoul "Diffraction phase microscopy: retrieving phase contours on living cells with a wavelet-based space-scale analysis," Journal of Biomedical Optics 19(3), 036007 (10 March 2014). https://doi.org/10.1117/1.JBO.19.3.036007

Published: 10 March 2014

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