Simultaneous multiplane imaging of human ovarian cancer by volume holographic imaging

Gabriel V. Orsinger; Jennifer M. Watson; Michael Gordon; Ariel C. Nymeyer; Erich E. de Leon; Johnathan W. Brownlee; Kenneth D. Hatch M.D.; Setsuko K. Chambers; Jennifer K. Barton; Raymond K. Kostuk; Marek Romanowski

doi:10.1117/1.JBO.19.3.036020

27 March 2014 Simultaneous multiplane imaging of human ovarian cancer by volume holographic imaging

Gabriel V. Orsinger, Jennifer M. Watson, Michael Gordon, Ariel C. Nymeyer, Erich E. de Leon, Johnathan W. Brownlee, Kenneth D. Hatch M.D., Setsuko K. Chambers, Jennifer K. Barton, Raymond K. Kostuk, Marek Romanowski

Author Affiliations +

Journal of Biomedical Optics, Vol. 19, Issue 3, 036020 (March 2014). https://doi.org/10.1117/1.JBO.19.3.036020

Abstract

Ovarian cancer is the most deadly gynecologic cancer, a fact which is attributable to poor early detection and survival once the disease has reached advanced stages. Intraoperative laparoscopic volume holographic imaging has the potential to provide simultaneous visualization of surface and subsurface structures in ovarian tissues for improved assessment of developing ovarian cancer. In this ex vivo ovarian tissue study, we assembled a benchtop volume holographic imaging system (VHIS) to characterize the microarchitecture of 78 normal and 40 abnormal tissue specimens derived from ovarian, fallopian tube, uterine, and peritoneal tissues, collected from 26 patients aged 22 to 73 undergoing bilateral salpingo-oophorectomy, hysterectomy with bilateral salpingo-oophorectomy, or abdominal cytoreductive surgery. All tissues were successfully imaged with the VHIS in both reflectance- and fluorescence-modes revealing morphological features which can be used to distinguish between normal, benign abnormalities, and cancerous tissues. We present the development and successful application of VHIS for imaging human ovarian tissue. Comparison of VHIS images with corresponding histopathology allowed for qualitatively distinguishing microstructural features unique to the studied tissue type and disease state. These results motivate the development of a laparoscopic VHIS for evaluating the surface and subsurface morphological alterations in ovarian cancer pathogenesis.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

Citation Download Citation

Gabriel V. Orsinger, Jennifer M. Watson, Michael Gordon, Ariel C. Nymeyer, Erich E. de Leon, Johnathan W. Brownlee, Kenneth D. Hatch M.D., Setsuko K. Chambers, Jennifer K. Barton, Raymond K. Kostuk, and Marek Romanowski "Simultaneous multiplane imaging of human ovarian cancer by volume holographic imaging," Journal of Biomedical Optics 19(3), 036020 (27 March 2014). https://doi.org/10.1117/1.JBO.19.3.036020

Published: 27 March 2014

ACCESS THE FULL ARTICLE

JOURNAL ARTICLE
11 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY