Optical clearing at cellular level

Matti Kinnunen; Alexander V. Bykov; Juho Tuorila; Tomi Haapalainen; Artashes V. Karmenyan; Valery V. Tuchin

doi:10.1117/1.JBO.19.7.071409

10 March 2014 Optical clearing at cellular level

Matti Kinnunen, Alexander V. Bykov, Juho Tuorila, Tomi Haapalainen, Artashes V. Karmenyan, Valery V. Tuchin

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Journal of Biomedical Optics, Vol. 19, Issue 7, 071409 (March 2014). https://doi.org/10.1117/1.JBO.19.7.071409

Abstract

Strong light scattering in tissues and blood reduces the usability of many optical techniques. By reducing scattering, optical clearing enables deeper light penetration and improves resolution in several optical imaging applications. We demonstrate the usage of optical tweezers and elastic light scattering to study optical clearing [one of the major mechanisms—matching of refractive indices (RIs)] at the single particle and cell level. We used polystyrene spheres and human red blood cells (RBCs) as samples and glycerol or glucose water solutions as clearing agents. Optical tweezers kept single microspheres and RBCs in place during the measurement of light scattering patterns. The results show that optical clearing reduces the scattering cross section and increases g] . Glucose also decreased light scattering from a RBC. Optical clearing affected the anisotropy factor g of 23.25-μm polystyrene spheres, increasing it by 0.5% for an RI change of 2.2% (20% glycerol) and 0.3% for an RI change of 1.1% (13% glucose).

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Matti Kinnunen, Alexander V. Bykov, Juho Tuorila, Tomi Haapalainen, Artashes V. Karmenyan, and Valery V. Tuchin "Optical clearing at cellular level," Journal of Biomedical Optics 19(7), 071409 (10 March 2014). https://doi.org/10.1117/1.JBO.19.7.071409

Published: 10 March 2014

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