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22 December 2015 Living cell dry mass measurement using quantitative phase imaging with quadriwave lateral shearing interferometry: an accuracy and sensitivity discussion
Sherazade Aknoun, Julien Savatier, Pierre Bon, Frédéric Galland, Lamiae Abdeladim, Benoit F. Wattellier, Serge Monneret
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Journal of Biomedical Optics, Vol. 20, Issue 12, 126009 (December 2015). https://doi.org/10.1117/1.JBO.20.12.126009
Abstract
Single-cell dry mass measurement is used in biology to follow cell cycle, to address effects of drugs, or to investigate cell metabolism. Quantitative phase imaging technique with quadriwave lateral shearing interferometry (QWLSI) allows measuring cell dry mass. The technique is very simple to set up, as it is integrated in a camera-like instrument. It simply plugs onto a standard microscope and uses a white light illumination source. Its working principle is first explained, from image acquisition to automated segmentation algorithm and dry mass quantification. Metrology of the whole process, including its sensitivity, repeatability, reliability, sources of error, over different kinds of samples and under different experimental conditions, is developed. We show that there is no influence of magnification or spatial light coherence on dry mass measurement; effect of defocus is more critical but can be calibrated. As a consequence, QWLSI is a well-suited technique for fast, simple, and reliable cell dry mass study, especially for live cells.
© 2015 Society of Photo-Optical Instrumentation Engineers (SPIE) 1083-3668/2015/$25.00 © 2015 SPIE
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Sherazade Aknoun, Julien Savatier, Pierre Bon, Frédéric Galland, Lamiae Abdeladim, Benoit F. Wattellier, and Serge Monneret "Living cell dry mass measurement using quantitative phase imaging with quadriwave lateral shearing interferometry: an accuracy and sensitivity discussion," Journal of Biomedical Optics 20(12), 126009 (22 December 2015). https://doi.org/10.1117/1.JBO.20.12.126009
Published: 22 December 2015
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Cited by 80 scholarly publications and 4 patents.
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KEYWORDS
Image segmentation
Phase imaging
Phase measurement
Objectives
Blood
Microscopes
Interferometry
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