CONFERENCE PROCEEDINGS
Advanced Search
Home > Journals > J. Biomed. Opt. > Volume 22 > Issue 12 > Article
Open Access
22 December 2017 Development and evaluation of a connective tissue phantom model for subsurface visualization of cancers requiring wide local excision
Kimberley S. Samkoe, Brent D. Bates, Niki N. Tselepidakis, Alisha V. DSouza, Jason R. Gunn, Dipak B. Ramkumar, Keith D. Paulsen, Brian W. Pogue, Eric R. Henderson
Author Affiliations +
Journal of Biomedical Optics, Vol. 22, Issue 12, 121613 (December 2017). https://doi.org/10.1117/1.JBO.22.12.121613
Abstract
Wide local excision (WLE) of tumors with negative margins remains a challenge because surgeons cannot directly visualize the mass. Fluorescence-guided surgery (FGS) may improve surgical accuracy; however, conventional methods with direct surface tumor visualization are not immediately applicable, and properties of tissues surrounding the cancer must be considered. We developed a phantom model for sarcoma resection with the near-infrared fluorophore IRDye 800CW and used it to iteratively define the properties of connective tissues that typically surround sarcoma tumors. We then tested the ability of a blinded surgeon to resect fluorescent tumor-simulating inclusions with ∼1-cm margins using predetermined target fluorescence intensities and a Solaris open-air fluorescence imaging system. In connective tissue-simulating phantoms, fluorescence intensity decreased with increasing blood concentration and increased with increasing intralipid concentrations. Fluorescent inclusions could be resolved at ≥1-cm depth in all inclusion concentrations and sizes tested. When inclusion depth was held constant, fluorescence intensity decreased with decreasing volume. Using targeted fluorescence intensities, a blinded surgeon was able to successfully excise inclusions with ∼1-cm margins from fat- and muscle-simulating phantoms with inclusion-to-background contrast ratios as low as 2∶1. Indirect, subsurface FGS is a promising tool for surgical resection of cancers requiring WLE.
© 2017 Society of Photo-Optical Instrumentation Engineers (SPIE) 1083-3668/2017/$25.00 © 2017 SPIE
Citation Download Citation
Kimberley S. Samkoe, Brent D. Bates, Niki N. Tselepidakis, Alisha V. DSouza, Jason R. Gunn, Dipak B. Ramkumar, Keith D. Paulsen, Brian W. Pogue, and Eric R. Henderson "Development and evaluation of a connective tissue phantom model for subsurface visualization of cancers requiring wide local excision," Journal of Biomedical Optics 22(12), 121613 (22 December 2017). https://doi.org/10.1117/1.JBO.22.12.121613
Received: 21 April 2017; Accepted: 1 December 2017; Published: 22 December 2017
JOURNAL ARTICLE
 12 PAGES
DOWNLOAD PAPER SAVE TO MY LIBRARY
GET CITATION
Lens.org Logo
CITATIONS
Cited by 24 scholarly publications.
Advertisement
Advertisement
RIGHTS & PERMISSIONS
Get copyright permission  Get copyright permission on Copyright Marketplace
KEYWORDS
Luminescence
Tissues
Tumors
Imaging systems
Surgery
Blood
Cancer
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top